Ham's F12 Medium, with phenol red、HEPES，318-011

Ham's F-12 Nutrient Mixture was developed by Ham in 1969 based on the Ham's F-10 nutrient mixture. It is widely used as a basal medium for serum-free culture and is particularly suitable for single-cell culture and clonal culture under low serum conditions.

This medium contains a comprehensive range of amino acids, vitamins, inorganic salts and other essential components required for cell growth. It is free of proteins, lipids and any growth factors, so it must be supplemented with serum or serum‑free supplements prior to use.