TG-SDS（Tris-Glycine-SDS）Buffer，Liquid 10×，880-570-LL

Product Description:

WISENT TG-SDS (Tris-Glycine-SDS) buffer, available as a 10× liquid pack, is a fundamental reagent extensively used in molecular biology research.

TG-SDS buffer comprises three main components: Tris base, glycine, and SDS. Tris base serves as the buffering agent in TG-SDS buffer, maintaining a stable pH environment for protein electrophoresis. Glycine enhances the conductivity of the buffer, facilitating efficient protein migration through the gel matrix during electrophoresis. SDS, a detergent, denatures proteins by disrupting non-covalent interactions and conferring a negative charge on the polypeptide chains. This uniform negative charge-to-mass ratio allows for the separation of proteins based solely on size during electrophoresis. TG-SDS buffer is commonly used in SDS-PAGE, a widely employed technique for separating proteins according to their molecular weight. SDS-PAGE is utilized for protein analysis, characterization, and purification in various research fields, including biochemistry, molecular biology, and proteomics. Additionally, TG-SDS buffer is utilized in Western blotting, a technique used to detect specific proteins within complex mixtures.