ADVANCED™ 1 Step RT-PCR KIT

ADVANCED™ 1 STEP RT-PCR KIT，801-101

WISENT ADVANCED 1-Step RT-PCR MIX uses the latest developments in reverse transcriptase technology and buffer chemistry for efficient cDNA synthesis and PCR in a single tube. Proprietary small-molecule hot-start technology offers improved specificity and sensitivity compared to other methods. Our Taq HS DNA Polymerase performs consistently well on a broad range of templates, including both GC-rich and AT-rich ones. We use a thermostable reverse transcriptase working at 45°C to 55°C, along with an advanced RNase inhibitor. Take advantage of our proprietary hot-start technology for unrivalled detection of low copy number templates.

Ordering Information:

CAT.NO.	Quantity	Specification
801-101-ZR	50 REACTIONS	RT PCR - 1 STEP
801-101-AR	100 REACTIONS	RT PCR - 1 STEP
801-101-ER	500 REACTIONS	RT PCR - 1 STEP

REMARKS:

1.Primers should have a predicted melting temperature of around 60°C using default primer 3 settings.

2.Reverse transscription: an incubating temperature of 45°C . For 10 minutes is recommended except where region of interest contain high secondary structure incubation in which case up to 55°C might be used. For aplicons above 1 kb, the incubation time should be increased to 20 min.

3.Determine the optimal annealing temperature experimentally by performing a temperature gradient.

4.Optimal extension is achieved at 72°C. Optimal extension time depends on amplicon length and template. Normally 15 seconds per kilobase is used for amplification for aplicons between 1 kb and 3 kb.

Product Description:

Product Stability And Storage:

On arrival store at -25°C to -15℃.

Avoid prolonged exposure to light.

If stored correctly the mix will retain full activity for 12 months. It is stable at 2-8°C for one month and can withstand 30 freeze/thaw cycles without any loss of activity.